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Differential interference contrast (DIC) microscopy is a specialized technique used in the study of live and unstained animal and human tissue samples in medical science. The accuracy of the system relies on the spatial uniformity of its many parts. Due to this, active vibration control is necessary to ensure image accuracy.
DIC microscopy is a method of optical microscopy illumination that enhances contrast in transparent specimens. Polarized light is directed into a modified Wollaston prism to split the light into two separate beams. One of the beams is directed towards the specimen, while the other only passes through the background. Both beams are recombined through another Wollaston prism, located above the microscope's objective lens.
This technique is able to produce contrast that visually displays the gradient of optical paths in every part of the specimen. The resulting image is monochromatic and has a three-dimensional appearance.
The components of a DIC microscopy system, including the light source, stage, and prisms, must maintain uniform distance to ensure the clarity of the image. Subtle vibrations coming from the central HVAC unit, equipment in adjacent rooms, and traffic along the hallway can affect the system and decrease the quality of results. Noise from outside the building and the equipment present in the lab also produce vibrations.
Active vibration cancellation systems are necessary to ensure the stability of DIC microscopy systems. These advanced systems utilize piezoelectric sensors and actuators to detect incoming vibrations and react by either tuning isolators to minimize vibrations or sending a signal to cancel them out. The system also creates a feedback loop that dampens inherent resonances in mechanical structures. Active systems can be combined with passive isolation systems, such as stiff metal springs for load support, to isolate a broad range of frequencies.
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